pGLO lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB carpet Yellowish Tan Greenish Blue - pGLO LB/amp none + pGLO LB/amp 164 Yellowish Tan Greenish Blue + pGLO LB/amp/ara 59 Yellowish Tan Greenish Blue

2.	What two new traits do your transformed bacteria have?
	Two new traits my transformed bacteria have are ampicillin resistance and arabinose.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	There were probably only one or two colonies of bacteria in the 100 uL of bacteria that was spread on each plate because when we got the bacteria with the lobe we only got a tiny amount of it.
4.	What is the role of arabinose in the plates?
	The role of arabinose in the plates was to activate the gene so that it could glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	In cellular biology, GFP has been used as: Reporter Gene (a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria cell culture, animals or plants) Cell Maker Fusion Tag (a protein or a peptide located either on the C- or N- terminal of the target protein)
6.	Give an example of another application of genetic engineering.

Genetic engineering is also used in agriculture to create genetically-modified crops or genetically-modified organisms.